Carbohydrate Polymers, 68, 477-488. 2000;3(9):31826. Weissella: An Emerging Bacterium with Promising Health Benefits. In fact, short chains do contain minor branching points. ; Tsuchiya, H.M.; Rist, C.E. A similar combination involves the intrinsic viscosity and specific volume: (a/b) is called Einstein viscosity increment, and Vs is specific volume (cm3/g). Sung-Wook Choi. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. (1991). Global hydrodynamic analysis of the molecular flexibility of galactomannans. Human skins (Full-thickness human skin) were supplied by HansBiomed. Carbohydrate Research, 160, 205-214. Critical parameters for gel permeation Kasaai, Mohammad R. (2007). The equation 4 can be rearranged and resulted in a modified M-H equation as follows: where the value qM-H is the polydispersity correction factor, and it is a statistical function of the molecular weight distribution. The skin was treated with the RF-based microporation device, inducing micropore ablation through alternating electrical current. The relation between Mw and the intrinsic viscosity is given by Mark-Houwink equation. Biotechnology Progress, 19, 544-548. This conjecture found to be correct. government site. Google Scholar. Pharm Res. Chapter 2.1.4 Suspended level viscometers for transparent liquid, Ubbelohde Viscometer page 28-31. Citation-(RIS
The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Soc., 16, 3A, 426-433. 43-1, 115-147. In the case of the work performed in references [34, 35, 36] they studied using the number average molecular weight, which rearranges the value of a and forces it to enter into unbranched or linear molecules, on the other hand, if molecular weights are used the value would be less than 0.5. The dextran was chosen as another branched polysaccharide which, like the others was suspected to show hyperbranched behavior. (2004). 2010;4(1):117. In vivo iontophoretic delivery of salmon calcitonin across microporated skin. HHS Vulnerability Disclosure, Help Middelkoop E, Bogaerdt AJ, Lamme EN, Hoekstra MJ, Brandsma K, Ulrich MMW. WebTry out PMC Labs and tell us what you think. The hydrodynamic radius (Rh) of individual samples of dextran, polyvinylpyrrolidone, and polyoxyethylene over a range of molecular weights (1,500 Surface morphology study of dextran by scanning electron microscopy showed the porous web like structure. (2008). Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. Ibrahim O, Munavalli GS, Dover JS. Progress in Measurement, Interpretation and Application to Structure in Dilute Solution. Springer Nature. Pathan IB, Setty CM. WebFITC Products. These hydrodynamic parameters show that these polysaccharides behave as a compact rigid sphere and contract by the increase of temperature (RH decreases) for the Mw range from 8.8 to 200kDa. Effect of temperature on the intrinsic viscosity and conformation of chitosan in dilute HCl solution. Huggins, Maurice L. (1942). The skin poration was performed using the microporation device (LG electronics, Korea). For a limited temperature range, the viscosity of a polymer solution varies generally following a relation similar to that of usual liquids (rrhenius plot), where the polymer concentration is not very high and that temperature remains far enough from glass transition [38], and for system PEAorganic solvents see reference [63]. "Dextrans in Aqueous Solution. Singh TRR, Garland MJ, Cassidy CM, Migalska K, Demir YK, Ryan SAE, Woolfson D, Donnelly RF. For Research Use Only. 2023 Feb 17;12(4):861. doi: 10.3390/foods12040861. On the molecular weight distribution of dextran T-500. The a value is higher than calculated in this work, because these calculations were performed with number average molecular weight (Mn) which rearranges the value of a and forces him to enter into unbranched or linear molecules (a > 0.5), on the other hand, if molecular weights (Mw) ware used to value would be less than 0.5. Web54 we estimated that the hydrodynamic radii of 3-5 kDa dextran, 20 kDa dextran, and 70 kDa dextran are 2 55 nm, 5 nm, and 6 nm, respectively. Webprotein hydrodynamic radius was observed with increasing protein concentration (in the range 0.25-2 mM) in both the absence and presence of 8 M urea. Learn More. These studies were again focused on investigating the hydrodynamic properties of the polymer molecules, their hydrodynamic radius in relation to their radius of gyration and the coherence of the simulation results with polymer theory. Webdextran hydrodynamic radius. The values of 0 decreases from 2.5429 to 2.3326 x 1023mol-1 demonstrating a relative flexibility of particles. After the pore closure examination, each sample was stored in a 10% neutral buffered formalin awaiting haematoxylin/eosin (H&E) staining. Arond L.H. For a rigid or rod like polymer molecule that is greatly extended in solution, the Mark-Houwink a constant approaches to a value of 2.0 [48].The hydrodynamic radius (RH), for a sphere ((a/b) = 2.5) is given by the Einstein relation [49]. Expert Rev Med Devices. Figure1 shows a schematic illustration of transdermal delivery after the application of the microporation device. FITC-derivatives have been used for a variety of biological applications for more than 40 yeras. Cite this article. A novel correlation for protein diffusion coefficients based on molecular weight on radius of gyration. By using this website, you agree to our Guner, A.; Kibarer, G. (2001). Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. Mitragotri S. Mechanical disruption of skin barrier for vaccine delivery. ; Bataille, B. (2013). We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Castellanos Gil, E. E.; Iraizoz Colarte, A.; El Ghzaoui, A.; Durand, D.; Delarbre, J.L. (2008). 2022 Feb 22;16(2):1999-2012. doi: 10.1021/acsnano.1c07010. Mark-Houwink-Kuhn-Sakurada Exponent at the Condition. ; Picout, D.R. Porcine wound models for skin substitution and burn treatment. European Biophysical Journal, 36, 85-94. NCI CPTC Antibody Characterization Program. Seven dextrans, provided by Sigma, with molecular weight of 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa. Superior prebiotic and physicochemical properties of novel dextran from Weissella cibaria JAG8 for potential food applications. Article The M-H equation is empirical and valid for monodisperse polymers, and it is applied to biomacromolecules (complex, hyperbranched, crosslink), taking the precaution of comparing the value of Mv with the polydispersity index (qM-H). The Hydrodynamic Radii of Macromolecules and Their Effect on Red Blood Cell Aggregation. 20K. * shows a significant difference between the M.W. Challenges in analysis of high-molar mass dextrans: Comparison of HPSEC, AsFlFFF and DOSY NMR spectroscopy. The skin culture medium was purchased from Biopredic International (Saint-Grgoire, France). Im Tiefkhlgert (-5 bis -30C) lagern und vor Licht schtzen. Saudi Pharm J. The enhanced permeability through micropores was confirmed by Franz diffusion cell. WebThe study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. The dextran has the advantage in terms of low cytotoxicity, excellent biocompatibility, and water-solubility. Colloid Polym. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Transdermal diffusion cell system (DHC-6TD, LOGAN instrument, USA) was used to assess FITC-dextran permeability in vitro. Fixable: Fixable via Lysine. The explanation for the anomalous values of a and kM-H is because the mixture solute-solvent is highly compatible due a great compaction of biopolymer, from undisturbed state in the absence of interactions, where free energy of mixing less than zero, and emphasize the nature non-linear or hyperbranched of dextran for Mw high to 200kDa. Catiker, E.; Guner, A. Journal of Colloid Science, 31, 308-328. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. These modifications include degradation of hemidesmosomes attached to the dermis, degradation of desmosomes linked to adjacent cells, contraction of intracellular tonofilament, and lamellipodia formation. For a values from 0-0.5 a rigid sphere is predicted in a ideal solvent, from 0.5-0.8 a random coil in good solvent, and from 0.8-2 a rigid or rod like shape is expected (stiff chain). Biomaterials, 22, 1891-1898. The solutions were prepared in bidestilated water at 1, 0.75, 0.5, 0.25 % wt. ; Yevlampieva N.P. Soc., 21, 1, 78-86. The mechanism of the dextran-induced red blood cell Aggregation. Herwadkar A, Banga AK. ; van Nostrum, C.F. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. Carbohydrate Research, 340, 811-826. Formation of dextran hydrogels by crystallization. Chromatographic characterization of polymers: Hyphenated and multidimensional techniques. Drug Deliv. Pore closure examination was performed using an optical microscope (BX43, Olympus, Japan) immediately (time 0), 4, 8, and 24h following device application. Molecular weight determination of polysaccharides. The Dextrans intrinsic viscosity is determined as a function of the molecular weight in water and NaOH 0.5 M see reference [1]. Federal government websites often end in .gov or .mil. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was This method utilizes promising strategies that have made a significant impact in transdermal delivery [6], they include: iontophoresis, microneedles, sonophoresis, radiofrequency (RF), and chemical enhancers [7,8,9,10,11]. Peptide and protein transdermal drug delivery. Differences were considered statistically significant at p <0.05. B 113, 8020-8025. De Belder, A. N. (2001). Walkenhorst, S.; Olivier, R. (2002). The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. HSE and DK helped to porate the skin and to measure TEWL. Journal of American Chemical Society, 76 (20), 5041-5052. A long-chain polymer molecule in solution takes on a somewhat kinked or curled shape, intermediate between a tightly curled mass (coil) and a rigid linear configuration. In this work, the human cadaver skin was treated to confirm the skin regeneration after the application of the microporation device. Transdermal drug delivery: overcoming the skins barrier function. Optical properties of dextran in solution and in films. The receptor chamber was filled with PBS. Amersham Biosciences, AA Edition, www.amershambiosciences.com. Biophysical Journal, 27, 177-181. 56 The linkage that the enzyme dextranase breaks down is indicated by the black arrow. 4h after microporation, the sizes of the micropore decreased slightly. An undergraduate physical chemistry experiment. FITC-dextran solution (600L) was added to the donor chamber. Viscosity of in water solution polysaccharides depends on intrinsic characteristics of the biopolymer (such as molecular weight, volume, size, shape, surface charge, deformation facility, esterification degree, and galacturonic content) and on ambient factors (such as pH, temperature, ionic strength, solvent, etc.). Changes in the Mark-Houwink hydrodynamic volume of chitosan molecules in solutions of different organic acids, at different temperatures and ionic strengths. After 24h the micropores were either closed or reduced further to smaller sizes, 37.5020.41, 50.5718.07, and 58.5814.99m, for 2, 5, and 10 times microporation, respectively. In this study, we investigated the effectiveness of RF microporation technology in vitro, by evaluating the permeation of fluorescent-conjugated dextran (FITC-dextran) of various molecular weights through the human and pig skin after microporation, using a transdermal diffusion cell system. High Manufacturing Standards of Molecular Probes Dextrans. Aqueous solutions of amphiphilic polysaccharides: Concentration and temperature effect on viscosity. (1990). Data File Dextran. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. Carbohydrate Polymers 88 373-381. Carbohydrate Polymers, 79, 380-390. Actually, everything depends on solvent you want to use and temperature. If you want to know gyration (Rg)or hydrodynamic (Rh) radius, you should (1969). Tirtaatmadja, V.; Dunstan, D. E.; Boger, D. V. (2001). The Mark-Houwink parameters is calculated molecular weights range from 8.8 to 200kDa. Determination of branching in biopolymers by size exclusion chromatography with light scattering (Malls), viscosity, and refractive index detection. Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation. Viscosity of Liquids. Ed., 9(2), 85-90. However, the results did not show significant regeneration of micropore compared to previous in vivo studies which reported significant regeneration [25]. Hi Khanh, Alan is taking a little time off of Research Gate at the moment, but here is some information in reply to your question. WebThe Stokes radius or StokesEinstein radius of a solute is the radius of a hard sphere that diffuses at the same rate as that solute. Although we didnt conduct assay for inflammation, we could not find any inflammation symptoms such as redness, blisters, cracked, and thickening. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. When applied to adherent cell cultures, the APGD-t is capable of inducing temporary cell permeabilization. ; Ortega, A.; Garcia de la Torre, J.; Harding, S.E. Anal. 2015;32(5):170413. volume24, Articlenumber:22 (2020) Interaction Parameter (); Expansion Factor (); Steric Hindrance Factor (); and Shielding Function F(); for the System PEAOrganic Solvents by Intrinsic Viscosity Measurements. ACS Nano. In addition, microporated pig skin permeated larger amounts of FITC-dextran than human skin, this can be attributed to the differences in structure between the human and pig skin; the pig skin layer is thinner (SC, 813m) than the human skin (SC, 1017m) and the pig hair follicle is longer (3871m) than the human hair follicle (18m). The micropore formed at the SC through to the epidermis, enabling drug delivery. For simplicity, macromolecules are treated as rigid molecules for a hydrodynamic study. Radiofrequency is a well-known medical technique with expanded application in electrosurgery for laparoscopic medical procedures and in dermatology, for sebaceous glands ablation in acne treatment [12, 13], and treatment of photoaging skin symptoms, including wrinkles and hyperpigmentation [14]. In summary, we demonstrated effective enhancement of skin permeability and delivery of macromolecules lower than 20KM.W., and regeneration of human cadaver skin after microporation with an RF-based microporation device. 2004;25(9):155967. Hrabchak C, Flynn L, Woodhouse KA.